Higher performance derived from an advanced optical system provides lower limit of detection, enhanced fluorescence performance and efficiency, a clear image to lessen operator dependence, and superior reproducibility. Greater ease of use comes from understanding user needs, including a built-in sample pump, sealed sample cell, bubble-free fluid transfer, and a stable optical system. The Envision was engineered to address the frustrations of researchers limited by the first generations of NTA instruments. By applying proven science and dedicated development, every aspect of the system has been improved so the analytical capabilities and the user experience are truly a generation ahead..

Advanced Optical Design

The Envision optical system delivers a high signal-to-noise image, enabling accurate and precise measurements across diverse sample types without background optical interference. This sharply focused imaging capability enhances sensitivity, expanding the NTA technique’s measurement range to smaller particle sizes than previously possible.

Furthermore, the optical engineering is precise and reproducible, so results will be comparable between instruments and laboratories. The image quality, measurement settings, and sample results are now consistent, showing minimal variation compared to the significant discrepancies observed with other NTA instruments.

38 nm Polystyrene Latex Beads

Breakthrough Lower Limit of Detection

High-quality imaging enables exceptional performance. The superior signal-to-noise ratio facilitates measurement of particles smaller than previously possible with NTA. The lower size detection limit varies with material refractive index, yet the minimal noise in imaging enables precise tracking of particles with weak scattering properties.This allows measurement of the full size range of extracellular vesicles (EV) or exosomes, smaller-sized liposomes and drug delivery nanoparticles, or smaller viruses than would be seen with competitive systems.

Fluorescence Stability

“The sensitivity of the optical system allows for much lower laser intensity to be used even for the smallest and dimmest particles, resulting in much improved stability of even photo-sensitive fluorescent labels.​​ Reducing laser intensity for fluorescence-mode NTA measurements means that fluorophores are only minimally bleached. In contrast to other systems where fluorescent particles typically exhibit short lifespans of a few seconds, the Envision consistently allows for prolonged observation of these samples over tens of seconds to minutes. This capability ensures robust and dependable measurement of all fluorescent samples, providing comprehensive data representation.”

Vesi-Ref EV CD63 GFP under
Scatter and Fluorescence Mode

Ease of Use

Software built around user workflow and automating the user dependent variables through the use of advanced technology means sample operation is a breeze, thus removing operator variables and improving throughput and repeatability even further. The built-in sample pump ensures consistent, bubble-free sample loading, repeatable flow speeds, and easy cleaning cycles through a fully automated SOP. The sealed sample cell is proven bubble-free and easily replaceable when needed for maximum up-time.

Robust, Reproducible, and Repeatable

By focusing on the collection of statistically significant populations, our workflow enhances reproducibility and repeatability by measuring a greater number of particles under optimal optical conditions. This approach significantly reduces inter-operator and inter-laboratory variability, leveraging increased statistical sampling to deliver stable results. Through consistent sample volumes, optimized optics for enhanced accuracy, and minimized user-dependent variables, we achieve improved repeatability in both size and concentration measurements.

44 nm Polystyrene Latex: 10 Replicate Runs

500 nm Silica Beads

Extended Dynamic Range

By expanding the lower limit while maintaining the capability to measure larger particles, the technique effectively broadens the dynamic range of the system. The robust construction of the optical system delivers clear and precise imaging, facilitating optimal conditions for a diverse range of sample types without compromise. Furthermore, a meticulously engineered vibration isolation system mitigates environmental influences on results, particularly in larger size ranges. This setup enables reliable measurement of larger samples made from various materials, with conditions tailored for each specific sample.

Quality Parts and Robust Design for Confident Reliability

Rather than optimizing design solely based on cost, the Envision was engineered with a primary focus on performance and reliability. Components were meticulously selected based on rigorous specifications and design criteria, prioritizing collaboration with top-tier vendors renowned for superior quality. Key design objectives encompassed durable components, long-term reliability, and minimized servicing costs. This commitment to long-term value underpins our confidence in offering a three-year warranty on laser modules and a two-year warranty on all other components.

Options (in development)

  • Online system for continuous monitoring of a process
  • Multi-laser system
  • Multi-camera system
  • Autosampler